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Medically reviewed by Drugs.com. Last updated on June 12, 2020.

Patch testing is the safest way to test whether your client is prone to a skin reaction called ‘contact dermatitis’. This is a type of skin sensitivity that can be triggered when the skin comes into direct contact with certain ingredients. It often results in itching, dryness and irritation that can also cause swelling. The terms Mantoux, TB skin test, tuberculin skin test, and PPD are often used interchangeably. Mantoux refers to the technique for administering the test. Tuberculin (also called purified protein derivative or PPD) is the solution used to administer the test. The preferred term for the test is tuberculin skin test or TST. Criteria Prescribed. The importance of skin testing prior to hair colouring. It is important that all our clients undergo a simple colour sensitivity test at least 48 hours prior to their first time colour application, or if they have not had colour with us for more than three months.Skin testing is here to protect you.Just pop into the salon and ask for a skin test. The nurse is conducting allergy skin testing on a client. Which postprocedure interventions are most appropriate for the nurse to perform? Select all that apply. Record site, date, and time of the test. Give the client a list of potential allergens if identified. Estimate the size of the wheal and document the finding. Handling Your Client's Allergic Reaction To A Skin Treatment When a client calls about an unusual reaction, a cell phone picture is nice, but a live check-in is better. Persuade them that you need to see them in person to get the best understanding of what has happened to their skin and to interview them about the reaction.

  • Professional


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Index Terms

  • Mantoux
  • PPD
  • TB Skin Test
  • TST
  • Tuberculin Purified Protein Derivative
  • Tuberculin Skin Test

Dosage Forms

Excipient information presented when available (limited, particularly for generics); consult specific product labeling.

Solution, Intradermal:

Aplisol: 5 units/0.1 mL (1 mL, 5 mL) [latex free; contains phenol, polysorbate 80]

Tubersol: 5 units/0.1 mL (1 mL, 5 mL) [contains phenol, polysorbate 80]

Brand Names: U.S.

  • Aplisol
  • Tubersol

Pharmacologic Category

  • Diagnostic Agent


Tuberculosis results in individuals becoming sensitized to certain antigenic components of the M. tuberculosis organism. Culture extracts called tuberculins are contained in tuberculin skin test preparations. Upon intracutaneous injection of these culture extracts, a classic delayed (cellular) hypersensitivity reaction occurs. This reaction is characteristic of a delayed course (peak occurs >24 hours after injection, induration of the skin secondary to cell infiltration, and occasional vesiculation and necrosis). Delayed hypersensitivity reactions to tuberculin may indicate infection with a variety of nontuberculosis mycobacteria, or vaccination with the live attenuated mycobacterial strain of M. bovis vaccine, BCG, in addition to previous natural infection with M. tuberculosis.

Onset of Action

Delayed hypersensitivity reactions: 5-6 hours; Peak effect: 48-72 hours

Duration of Action

Reactions subside over a few days

Use: Labeled Indications

Tuberculosis skin test: An aid in the diagnosis of tuberculosis (TB) infection.


Hypersensitivity to tuberculin purified protein derivative (PPD) or any component of the formulation; previous severe reaction to tuberculin PPD skin test (TST); documented active TB or clear history of treatment for TB infection or disease; extensive burns or eczema.

Dosing: Adult

Note: Dosing below is based on US/Canadian products which contain the PPD-S formulation; in regions outside of North America other products may be used, including the RT 23 formulation, and standard doses may differ between products as the units are not equivalent (Yang 2012).

Tuberculin skin test: Intradermal: 5 units (0.1 mL)

TST interpretation: Criteria for positive TST read at 48 to 72 hours (see Note below for health care workers; CDC 2000):

Induration ≥5 mm: Patients with HIV infection (or risk factors for HIV infection, but unknown status), recent close contact to person with known active TB, patients with chest x-ray consistent with prior TB, patients with organ transplants and other immunosuppressed patients (receiving the equivalent of prednisone ≥15 mg/day for ≥1 month

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Induration ≥10 mm: Patients with clinical conditions that increase risk of TB infection, recent immigrants (ie, ≤5 years) from high-prevalence countries, IV drug users, residents and employees of high-risk congregate settings, mycobacteriology laboratory workers, children <4 years of age, or infants, children, and adolescents exposed to adults at high risk

Induration ≥15 mm: Patients who do not meet any of the above criteria (no risk factors for TB)

Note: A two-step test is recommended when testing will be performed at regular intervals (eg, for health care workers). If the first test is negative, a second TST should be administered 1 to 3 weeks after the first test was read.

TST interpretation (CDC guidelines) in a health care setting (CDC 2005):

Baseline test: ≥10 mm is positive (either first or second step); 0 to 9 mm is negative

Serial testing without known exposure: Increase of ≥10 mm is positive

Known exposure:

≥5 mm is positive in patients with baseline of 0 mm

≥10 mm is positive in patients with negative baseline or previous screening result of >0 mm

Dosage adjustment for concomitant therapy: Significant drug interactions exist, requiring dose/frequency adjustment or avoidance. Consult drug interactions database for more information.

Dosing: Geriatric

Refer to adult dosing.

Dosing: Pediatric

Tuberculin skin test: Intradermal: Infants ≥6 months, Children, and Adolescents: Refer to adult dosing.


For intradermal administration only; do not administer IV, IM, or SubQ. Administer to upper third of forearm (palm up) ≥2 inches from elbow, wrist, or other injection site. If neither arm can be used, may administer to back of shoulder (CDC 2005). Avoid skin that is red or swollen; avoid visible veins. Administer using 1/4- to 1/2-inch 27-gauge needle or finer tuberculin syringe (CDC 2005). Should form wheal (6 to 10 mm in diameter) as liquid is injected which will remain ~10 minutes. Avoid pressure or bandage at injection site. Should wheal fail to form, repeat the test immediately at another site, at least 2 inches from the first site and circle the second injection site as an indication that this is the site to be read. Document date and time of injection, person placing TST, location of injection site, and lot number of solution. Read test at 48 to 72 hours following placement. Test results should be documented in millimeters even if classified as negative. Erythema and redness of skin are not indicative of a positive test result.


Store at 2°C to 8°C (36°F to 46°F); do not freeze. Protect from light. Opened vials should be discarded after 30 days.

Drug Interactions

Vaccines (Live): May diminish the diagnostic effect of Tuberculin Tests. Management: If a parenteral live vaccine has been recently administered, do administer a scheduled PPD skin test for at least 4-6 weeks following administration of the vaccine. Simultaneous administration of a parenteral live vaccine and PPD skin test is acceptable. Consider therapy modification

Test Interactions

False-positive reactions may occur with BCG vaccination or previous mycobacteria (non-TB) infection (previous BCG vaccination is not a contraindication to testing; however, an interferon gamma release assay is preferred [CDC 2010]). False-negative reactions may occur with any condition that impairs or attenuates cell-mediated immunity, including aging.

Adverse Reactions

The following adverse drug reactions and incidences are derived from product labeling unless otherwise specified.

Frequency not defined:

Cardiovascular: Presyncope, syncope

Dermatologic: Erythematous rash, localized erythema, localized vesiculation, rash at injection site, skin rash, skin ulceration at injection site, urticaria at injection site

Hypersensitivity: Anaphylactoid reaction, anaphylaxis, angioedema, hypersensitivity reaction

Local: Injection site reactions, discomfort at injection site, hematoma at injection site, injection site scarring, local pruritus, localized edema, local tissue necrosis, pain at injection site

Respiratory: Dyspnea, stridor

Miscellaneous: Fever


Disease-related concerns:

• Viral infections: Skin testing may be deferred with major viral infections.

Special populations:

• Pediatric: Infants <6 months of age may have an absent or delayed response.

Other warnings/precautions:

• Administration: For intradermal administration only; do not administer IV, IM, or SubQ. Epinephrine (1 mg/mL) should be available to treat possible allergic reactions.

• Appropriate use: Patients with a previous severe reaction to TST (vesiculation, ulceration, necrosis) at the injection site should not receive tuberculin PPD again.

• Conditions decreasing response: Tuberculous or other bacterial infections, fungal infections, viral infection, live virus vaccination, malignancy, corticosteroids or immunosuppressive agents, chronic renal failure, severe protein depletion, afibrinogenemia, and other conditions that impair immune response may cause a decreased response to test.

Monitoring Parameters

Monitor for immediate hypersensitivity reactions for ~15 minutes following injection.

Pregnancy Considerations

Tuberculin skin tests are valid and acceptable for use during pregnancy. Active tuberculosis infection is associated with adverse fetal outcomes, including intrauterine growth restriction, low birth weight, preterm birth, and perinatal death (Esmail 2018; Miele 2020), as well as adverse maternal outcomes, including increased risks for anemia and cesarean delivery. Placental transmission may rarely occur with active maternal disease (Miele 2020). An evaluation of tuberculosis risk factors and tuberculosis testing is recommended as part of antenatal care when indicated (Miele 2020).

Patient Education

What is this drug used for?

• It is used during a TB (tuberculosis) test.

WARNING/CAUTION: Even though it may be rare, some people may have very bad and sometimes deadly side effects when taking a drug. Tell your doctor or get medical help right away if you have any of the following signs or symptoms that may be related to a very bad side effect:

• Severe dizziness

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• Passing out

• Injection site burning, discoloration, pain, skin breakdown, or swelling

• Signs of an allergic reaction, like rash; hives; itching; red, swollen, blistered, or peeling skin with or without fever; wheezing; tightness in the chest or throat; trouble breathing, swallowing, or talking; unusual hoarseness; or swelling of the mouth, face, lips, tongue, or throat.

Note: This is not a comprehensive list of all side effects. Talk to your doctor if you have questions.

Consumer Information Use and Disclaimer: This information should not be used to decide whether or not to take this medicine or any other medicine. Only the healthcare provider has the knowledge and training to decide which medicines are right for a specific patient. This information does not endorse any medicine as safe, effective, or approved for treating any patient or health condition. This is only a limited summary of general information about the medicine’s uses from the patient education leaflet and is not intended to be comprehensive. This limited summary does NOT include all information available about the possible uses, directions, warnings, precautions, interactions, adverse effects, or risks that may apply to this medicine. This information is not intended to provide medical advice, diagnosis or treatment and does not replace information you receive from the healthcare provider. For a more detailed summary of information about the risks and benefits of using this medicine, please speak with your healthcare provider and review the entire patient education leaflet.

Further information

Always consult your healthcare provider to ensure the information displayed on this page applies to your personal circumstances.

More about tuberculin purified protein derivative

Consumer resources

Professional resources

Other brands:Tubersol, Aplisol

Related treatment guides

Why do I need to have a skin test? I have been colouring my hair for years!

This is a common statement that we as hair care professionals hear all the time from many new colour clients. If you are reading this blog you may be guilty of actually saying this yourself.

Why do we skin test?

Skin testing follows the manufacturer’s guidelines put in place for the usage of their hair products. It is a legal requirement that is put in place for the safety of all clients having a hair colour service, under Section 3 of the Health & Safety at Work Act.

Failure to patch test has led to many avoidable prosecution and fines in the past within the industry. So not only are hair salons putting their clients at risks but by not complying to this law they are also putting their business at risk.

We need to perform a skin test every time we have a new client. We also need to give skin tests if our clients want to use a different hair colour even within the same brand.

Prevention is better than a cure…

As a hair salon, to not give your client a skin test is negligence. All hair salons have a duty of care to all of their clients and this is one of them. Personally, if a hair salon said that they will colour my hair without following these basic procedures that will affect my wellbeing. I will not have confidence in them. I think it is wiser to sacrifice 48hrs waiting time for a skin test then months of recovery from a severe allergic reaction.

Allergic reactions to chemicals in hair dye can result in burning and scarring to the face and scalp, hair loss and even baldness in extreme cases. Allergies can easily be developed by repeat exposure, which means you can develop an allergy to hair colour at any time. Prevention is truly better than a cure…

Our body changes

I am absolutely mad about nuts! I used to eat them every day without fail. One day I could no longer eat them after a really bad reaction. After 2 years and many visits to the dermatologists. I am still unable to introduce them back into my diet without a horrible reaction. Nuts are just something that I will no longer have the privilege to enjoy. This is just an example of how an allergy or intolerance can develop from doing something repeatedly, or simply because of the natural changes within our body.

Skin testing applies to all

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Skin testing applies to permanent hair dye, organic natural hair dye or ammonia-free hair dye. Many believe that it is the ammonia that they have an allergic reaction too and that is actually false. You are more likely to have an allergic reaction to the pigments that are within the hair dye, in particular, a chemical called PPD (paraphenylenediamine), this ingredient is proven to cause allergic reactions in hypersensitive individuals, it is a known irritant and allergen. Hair dyes containing PPD are still safe to use. Providing that manufacturer’s safety instructions are followed.

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At Cognito Hair we value our clients, their health and well being is important to us. Therefore, we believe that we have a duty of care towards all our customers. This is why will always perform skin testing at least 48hrs prior to their hair colour appointment. We also offer ammonia-free hair colour options for those who simply don’t want to use ammonia because of the smell (as some feel it can be quite pungent) or for those who for various reason desire to move away from using ammonia.

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Come and see us at the hair salon for a complimentary consultation. Our hair colour experts will guide you into healthier coloured hair, and healthier scalp… until next time…

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19th March 2019, By Shareefa

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